Ovulation Midstream Test Instructions

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For a printable version of Ovulation Test instructions, please click here.
HOW IT WORKSHormones chart
Ovulation is the release of an egg from the ovary. The egg passes into the fallopian tube where it is ready to be fertilized. In order for pregnancy to occur, the egg must be fertilized by sperm within 24 hours after its release. Immediately prior to ovulation, the body produces a large amount of luteinizing hormones (LH). This is known as an “LH surge” and usually takes place in the middle of the menstrual cycle. LH triggers the release of an egg from the ovary. The BFP Ovulation Test helps predict the time of ovulation and peak fertility. It is during the fertile time that pregnancy is most likely to occur. The BFP Ovulation Test detects the LH surge in urine, indicating that ovulation is likely to occur in the next 12 to 36 hours. It is important to note that an LH surge and ovulation may not occur in all cycles.
Calculate when to start testing using the chart to the right. First calculate the length of your average menstrual cycle. Your menstrual cycle length is the number of days from the first day of your period (menstrual bleeding) to the last day before your next period starts. If your cycle is irregular, you may want to use your shortest cycle length.
EXAMPLE: Your usual cycle length is 28 days. Your period started on the 1st day of the month. The “When to Start Testing Chart” shows to begin testing on cycle day 11. Beginning with the 1st day, count ahead 11 days on the calendar. You would begin testing your urine on the 11th of the month.
Read all the information before performing the test. Do not open the foil pouch until you are ready to start the test. Not every woman ovulates at mid-cycle, therefore, you may not see a positive result during your first 5 days of testing. Continue testing with the BFP Ovulation Test to detect your LH surge. Some prescription drugs may affect the result such as menotropinls for injection (Pergonal®) and danazol (Danocrine®). If you are using Clomiphene Citrate (e.g. Clomid® and Serophene®), consult your physician for possible interference with the test. Some rare medical conditions and/or the onset of menopause can cause elevated LH levels. Some women do not ovulate every cycle and will not see an increase in LH levels during these non-ovulating cycles.

  1. First morning urine should NOT be used with this test. For best results, you should test around the same time each day. You should reduce your liquid intake approximately 2 hours prior to testing.
  2. Remove the test from the pouch just before testing. Remove the cap.
  3. Hold the test pointing downward directly into your urine stream and urinate onto the absorbant tip for at least 5 seconds. Do not urinate on the test control window. If you prefer, you can urinate into a clean dry container, and dip only the absorbent tip of the test into the urine for at least 5 seconds.
  4. Replace the cap and place the test on a flat surface. Read results between 5-10 minutes.

While you are waiting for your results, you may notice a colored solution moving across the windows. This is normal. You need to wait 5-10 minutes to read the results. Do not read the results after 30 minutes. To determine your results, compare the color intensity of the test line to the control line as follows:
Ovulation Comparison ChartPositive = LH Surge:
If 2 lines appear and the line in the test region (T) is similar to or darker in color intensity compared to the line in the control region (C), your LH has surged and you will likely ovulate in the next 12 to 36 hours.
Negative = No LH Surge:
If 2 lines appear and the line in the test region (T) is lighter in color intensity compared to the line in the control region (C), your LH level has not reached the threshold to fire a positive result. Also, if only one line appears in the test control window, your LH level has not reached the threshold to fire a positive result. You should continue daily testing.
If no line appears in the test control window, the result is invalid. Insufficient specimen volume or incorrect test performance is the most likely reason for an invalid result. This can be caused by not holding the absorbent tip in your urine for at least 5 seconds. If you receive an invalid result, urinate on the absorbent tip (or dip the absorbent tip into your urine) for an additional 5 seconds and wait 5-10 minutes. Read the results again according to the instructions. If there is still no line in the control window, the test is invalid. Review the instructions and then repeat with a new test.
Q: Can I use the BFP Midstream Ovulation Tests to avoid pregnancy?
A: No, the test should not be used as a form of birth control.
Q: How accurate is the BFP Midstream Ovulation Test?
A: In laboratory studies, the BFP Midstream Ovulation Test has been proved to be greater than 99% accurate.
Q: Do alcohol or medications affect the test?
A: No, but you should consult your physician if you are taking any hormonal medication. Also, recent oral contraceptive use, breastfeeding, or pregnancy could affect test results.
Q: Why should I not use first morning urine? What time of the day should I perform the test?
A: We do not recommend first morning urine because it is concentrated and may give a false positive result. Any other time of day is suitable. For best results, try to collect your urine at approximately the same time each day.
Q: Will the amounts of liquid I drink affect the result?
A: A heavy intake of fluids prior to testing will dilute the hormone in your urine. We suggest that you limit your fluid intake about two hours before you perform the test.
Q: How long will the line remain visible?
A: The test should be read at 5 - 10 minutes for best results. A positive result will never disappear. The color of the line may become darker and a tinted background may appear after several hours. Some negative results may later display a faint test line due to evaporation from the testing zone. Therefore, you should not read the results after 30 minutes and discard the test once you have read the result.
Q: Once I see a positive result, when is the best time to have intercourse?
A: Ovulation is likely to occur within 12 to 36 hours. This is your most fertile time. Sexual intercourse within this time frame is advised.
Q: I am now using the basal body temperature method (BBT). Does this test replace BBT?
A: The BFP Midstream Ovulation Test may replace the BBT method if you choose or may be used simultaneously. The shift in basal body temperature primarily indicates ovulation has already occurred. The BFP Ovulation Test Strip indicates that ovulation is about to occur.
Q: I received a positive result and had intercourse during these fertile days but I have not become pregnant. What shall I do?
A: There are many factors that can affect your ability to become pregnant. It can take normal, healthy couples many months to achieve a pregnancy and often you may need to use the kit for 3 to 4 months before achieving pregnancy. If pregnancy is not achieved after 3 to 4 months, you and your partner should consult a physician.

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